Effects of the Traditional Chinese Medicine Formula Ento-PB in Experimental Models of Ulcerative Colitis

The traditional Chinese medicine (TCM) formula Ento-PB containing Periplaneta americana (Linnaeus) (Blattidae) and Taraxacum mongolicum Hand.-Mazz. (Compositae) has great potential for treating inflammation. This study explored the effects of on ulcerative colitis (UC). UC model was induced with 2,4,6-trinitrobenzene sulfonic acid (TNBS) by enema. Male Sprague–Dawley rats (n = 32) were divided into four groups: (1) control group that received 2.5 mL/kg normal saline, (2) TNBS (3) low-dose 100 mg/kg Ento-PB, (4) high-dose 200 Ento-PB. Rats administered drugs via enema 14 days after modeling. disease activity index (DAI), colon mucosa damage (CMDI), histopathological score (HS), levels interleukin-8 (IL-8), IL-10, IL-17, tumor necrosis factor-α (TNF-α), C-reactive protein (CRP) in serum, contents IL-2, myeloperoxidase (MPO), transforming growth factor-β1 (TGF-β1), epidermal factor (EGF) colon, abundance Bifidobacterium, Lactobacillus, Enterococcus, Bacteroides, Escherichia coli assessed. administration showed a significant reduction DAI, CMDI, HS, IL-8, TNF-α, CRP, MPO, increase TGF-β1, EGF. Compared TNBS-administered group, E. decreased, while an obvious proportion Bacteroides found Ento-PB-administered groups. alleviated inflammation regulating equilibrium Th1/Th17/Treg cytokines recovering imbalance between gut microbiota. Applying could be suggested.